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Analyzing the Antibody Validation Survey from the GBSI

Sharon Craggs

Aug 30, 2016

Science 1

The GBSI decided to focus their conclusions on the customer side of the equation. The results of the survey identifying an urgent need to improve education and training of junior scientists in application-specific antibody validation as well as developing validation methodologies. However, manufacturer need to also provide more data and protocols for their products and suppliers need to work on ways to engender more trust with their customers.

Author: Sharon Craggs An experienced researcher and technical advisor with laboratory experience in academic and small biotechnology laboratories.

Sharon Craggs

GBSI Antibody Survey: our thoughts


The Global Biological Standards Institute created the Antibodies Task Force in 2014 consisting of antibody experts from academia and industry to try and improve the specificity, selectivity and reproducibility of research antibodies. One of the first recommendations from the Task Force was to create a survey to gather valuable information about how researchers view best practices regarding the use of antibodies in preclinical research and to identify challenges and opportunities to implementing those practices going forward.

The results of the survey were published in a Letter to the Editor of BioTechniques in July 2016 identifying an urgent need to:

1) improve the education and training of junior scientists in application-specific antibody validation;

2) develop validation methodologies and best practices that are rapid, simple, and cost effective, in part to counter an apparent perception of antibody validation delaying rather than facilitating research progress.

Looking at the Industry focused Questions

The full questionnaire and results are published for all to review here. What we found interesting about the Letter versus the survey questions and answers was the focus was on end users and not on manufacturers. Perhaps this was due to the need to focus on a particular headline or that the authors were mostly from academia – and there is undoubtedly a real need for those using antibodies to be properly trained and to make sure the antibodies they use are specific and work effectively in the experiments they are about to perform.

However, as a company that supports reagent manufacturers we looked at the results from an industry perspective and took the following takeaways in addition to those about end users:

  1. Manufacturers need to be more thorough in providing more data and protocols for their products
  2. Suppliers need to work on ways to engender more trust with their customers

The key questions and responses that led to these conclusions as follows:

Do your antibody vendors or other sources of antibodies provide guidance on usage of amount/concentration per application/assay?

Value   Percent Count
All provide guidance 15.80% 63
Most provide guidance 50.00% 199
Some provide guidance 31.20% 124
None provide guidance 1.30% 5
Unsure 1.80% 7
Total 398

If suppliers are going to say that an antibody has been tested in an application, they should be able to provide guidance on how to use the antibody in that application. 100% of antibody vendors should provide guidance.

Please rank the typical accuracy and reliability of such guidance.

Value Percent Count
Low 18.90% 73
Moderate 66.10% 255
High 12.70% 49
N/A 2.30% 9
Total 386

Again, suppliers need to do better: 85% of guidance is low to moderate in reliability. We can understand why 70% of respondents in the survey mentioned that antibody validation takes too much time if the researchers are having to run experiments to double check the reliability of supplier data.

Please rate how past performance (or lack thereof) of purchased antibodies influences your decision to purchase additional antibodies from a particular vendor:

Value        Percent Count
None 1.30% 5
Little 2.30% 9
Moderate 31.60% 126
Heavily 63.90% 255
Unsure 1.00% 4
Total 399

This result shows how difficult it is for new antibody firms to change the status quo: as long as researchers are happy with the past performance of their purchases they will not change supplier. This result also shows that the bad performance of one antibody will affect future sales of all antibodies to that customer: antibody manufacturers therefore need to make sure all of their antibodies are validated to the highest level.

Do you purchase antibodies based on vendor-supplied validation data (if available)?

Value Percent Count
Never 4.50% 18
Occasionally 40.90% 164
Routinely 49.90% 200
Unsure 4.70% 19
Total 401

In this result there is clearly a trust issue: over 45% of respondents try not to buy based on vendor supplied validation data. Suppliers need to work hard to prove that their data is worth trusting.

What type of vendor-supplied validation data do you look for and assess? Select all that apply:

Value Percent Count
Literature citations 79.30% 287
Application-specific data 75.70% 274
Western blot/ELISA data 74.00% 268
Tested in multiple cell lines 41.20% 149
Tested using native antigen 36.20% 131
Tested using recombinant antigen 24.60% 89

The majority of respondents are looking for outside data to prove the specificity / reliability of antibodies through literature searches. Antibody manufacturers might want to focus on validating their antibodies in Western blot and ELISA at the very least.


The GBSI decided to focus their conclusions on the customer side of the equation – Pivotal Scientific focused on the supplier side, but clearly we need to work to improve both sides to lead to lasting improvements to life science research as a whole. The other activities that the GBSI is undertaking such as their online discussions and workshop are a very real step in the right direction and Pivotal Scientific will work hard to support our Alliance members and the industry in general to make the required changes.

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